Author(s): Zolaikha Rasouli, Raouf Ghavami*.
Journal Name: Current Analytical Chemistry
Iodide (I-) is a vital micronutrient and its essential roles have been approved in a number of neurological activities. Therefore, there is a growing interest in developing reliable sensitive quantitative methodologies for the determination of the amount of I- in edible salt samples.
First, 2heDTC was synthesized by the mixing of equivalent amounts of pure C2H7NO and CS2 in the presence of NH4OH under vigorous stirring in an ice bath. Then, AuNPs were synthesized; the 250 mL of 1 mM HAuCl4 was put into a round-bottomed flask and was boiled vigorously by stirring for 20 min. Then, 25 mL of 38.8 mM of Na3C6H5O7 was added rapidly into this solution. The solution was heated under reflux for another 15 min. The modification of the AuNPs surface was performed with 2heDTC by proceeding with the ligand exchange reaction. To the quantification of I-, 2 µL (1mM) Hg2+ was added to 0.5 mL (50 mM) of PBS solution with pH = 5.0 containing 500 µL of 2heDTC-AuNPs, which was then maintained at optimized time for the creation of bare AuNPs. Then, the different concentrations of I- were separately added. Finally, the surface plasmon resonance (SPR) absorption bands were taken with UV-Vis spectrophotometer. Read out full article here: http://www.eurekaselect.com/161194