Author(s): Soheila Montazersaheb, Masoumeh Kazemi, Elahe Nabat, Peter E. Nielsen*, Mohammad S. Hejazi*.
Journal Name: Current Pharmaceutical Biotechnology
Antisense oligonucleotides are able to modulate splicing patterns and offer therapeutic intervention for cancer and other diseases. Considering TdT potential as a target in cancer therapy, the present study aimed to investigate splicing alteration of TdT pre-mRNA in Molt-4 cells using peptide nucleic acid (PNA) octaarginine and cholic acid conjugates.
We examined 16 mer PNAs targeting 5′ and 3′ junctions of intron 7 and addressed their mRNA splicing modulation effects using RT-PCR analysis. We also tested corresponding 2-base mismatch PNAs to confirm the sequence specificity. In addition, protien level of TdT, apoptosis induction and cell viability rate were analysed. Read out full article at: http://www.eurekaselect.com/169709/article