Study on Single-Molecule Biophysics and Biochemistry in Liquids and Live Cells: No immobilization/flow

Gerd Baumann, Zeno Foldes-Papp

What is it about?

How far apart do two molecules / two particles have to be in the time domain so that the required degree of separation between the two individual molecules / the two individual particles can be quantified at the molecular scale in order to distinguish them as separate entities without immobilization or hydrodynamic flow? Enjoy the real world of physics! Since mathematics provides a way to answer questions about the thermodynamic jitter in a clear, rational manner, with evidence to support it, mathematics is the reliable method necessary to get the best information on the movement of a single molecule / a single particle at the molecular scale in dilute liquids and live cells without immobilization or hydrodynamic flow.

Why is it important?

Földes-Papp’s thermodynamically temporal single-molecule limits, or Földes-Papp’s temporal resolution in diffraction limited and unlimited optical systems for dilute liquids or live cells without immobilization on a solid phase or membrane as well as without significant hydrodynamic flow,or even shorter, Földes-Papp’s limits are the reference measures (so called ‘golden standard’) for any temporal resolution calculations/algorithms based upon parameter sets of (specific) measurement techniques, for example, photon counting statistics, Nyquist limit, FRET, etc., in order to ultimately justify whether or not a single molecule/particle (individual molecule/particle, selfsame molecule/particle) was measured during the observation/detection time in dilute liquids or live cells without immobilization on a solid phase or membrane as well as without significant hydrodynamic flow. Földes-Papp’s limits are limits in measurement times that should not be exceeded when one wants to follow the selfsame molecule with high probability. Richard Levenson, Professor and Vice Chair for Strategic Technologies, Dept. at UC Davis, Davis, California, USA, wrote to me on May 24, 2021: ‘Much easier on Pluto, I imagine. A very cold, former planet. Not too much thermodynamic jitter at -233 C.’

Read now: https://bit.ly/3nrHUbe

New eBook Announcement | Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications

 

BY
ANUPAM JYOTI, NEETU MISHRA

Biomedical research is gaining prime attention as it is directly affecting human health. Biophysical, biochemical and cellular techniques are the backbone of biomedical sciences; a pre-requisite towards the understanding and treatment of human diseases. In the recent years, biomedical research has provided solutions for several problems faced by human beings. It is an integrated approach that includes various disciplines viz. biochemistry, microbiology, genetics and biochemical engineering. After some time, it has been integrated rapidly with new branches of science like molecular and cellular biology, genomics, proteomics, bioinformatics and nanotechnology. Biomedical research involves fundamental scientific principles applied to preclinical understanding of problems to clinical solutions. This book titled ‘Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications’, presents a broad overview regarding the basic applications of sophisticated and analytical techniques used in biophysics, biochemistry and cell biology. This book has covers the following areas: in vitro cell culture assay, real-time PCR, flow cytometry, and X-ray crystallography, discussed by authors who have quality publications in their proposed chapter area. Additionally each chapter includes application of techniques in specific areas like cells sorting by FACS, disease diagnosis by Real-time PCR, disease modeling under in vitro culture and many more. This will help students understand the importance of techniques in biophysics, biochemistry and cell biology research which will set a benchmark for further research.

As a biotechnology scientist, I am happy to recommend this book to the students of universities, both as a text and reference book. The section on technique will be used as textbook and the application section as reference. This book has been written in a way so that it is student-friendly with clean diagrams and protocols of specific techniques. I sincerely believe that the book has been prepared with the scientific skills and will serve as a useful document for the graduate and undergraduate students. Read out the full version here

 

Rajiv Kumar
ICAR-Central Sheep and Wool Research Institute
Avikanagar, Tonk, Rajasthan
India

Recent Issue of the BSP Journals have been Published

Current Pharmaceutical Design, 20 Issue 38

Aims & scope

Current Pharmaceutical Design publishes timely in-depth reviews and research articles from leading pharmaceutical researchers in the field, covering all aspects of current research in rational drug design. Each issue is devoted to a single major therapeutic area guest edited by an acknowledged authority in the field.

Each thematic issue of Current Pharmaceutical Design covers all subject areas of major importance to modern drug design including: medicinal chemistry, pharmacology, drug targets and disease mechanism.

http://www.eurekaselect.com/124887/issue/38

Abstracted & Indexed in

Biochemistry & Biophysics Citation Index®, BIOSIS, Chemical Abstracts, Current Contents®/Life Sciences, EMBASE/Excerpta Medica, MEDLINE/Index Medicus, Medical Documentation Service®, Research Alert®, Science Citation Index®, Science Citation Index Expanded (SciSearch®), Cambridge Scientific Abstracts (CSA), PubsHub, Journal Citation Reports/Science Edition, Index to Scientific Reviews®, Custom Information Services®, BIOSIS Previews, BIOSIS Reviews Reports and Meetings, Scopus, Genamics JournalSeek, and J-Gate.

For details about the latest issues, visit:http://benthamscience.com/journal/index.php?journalID=cpd

 

Current Pharmaceutical Design, 20 Issue 39

Aims & scope

Current Pharmaceutical Design publishes timely in-depth reviews and research articles from leading pharmaceutical researchers in the field, covering all aspects of current research in rational drug design. Each issue is devoted to a single major therapeutic area guest edited by an acknowledged authority in the field.

Each thematic issue of Current Pharmaceutical Design covers all subject areas of major importance to modern drug design including: medicinal chemistry, pharmacology, drug targets and disease mechanism.

http://www.eurekaselect.com/124928/issue/39

Abstracted & Indexed in

Biochemistry & Biophysics Citation Index®, BIOSIS, Chemical Abstracts, Current Contents®/Life Sciences, EMBASE/Excerpta Medica, MEDLINE/Index Medicus, Medical Documentation Service®, Research Alert®, Science Citation Index®, Science Citation Index Expanded (SciSearch®), Cambridge Scientific Abstracts (CSA), PubsHub, Journal Citation Reports/Science Edition, Index to Scientific Reviews®, Custom Information Services®, BIOSIS Previews, BIOSIS Reviews Reports and Meetings, Scopus, Genamics JournalSeek, and J-Gate.

For details about the latest issues, visit: http://benthamscience.com/journal/index.php?journalID=cpd

 

Cardiovascular & Hematological Disorders-Drug Targets, 14 Issue 3

Aims & scope

Cardiovascular & Hematological Disorders-Drug Targets aims to cover all the latest and outstanding developments on the medicinal chemistry, pharmacology, molecular biology, genomics and biochemistry of contemporary molecular targets involved in cardiovascular and hematological disorders e.g. disease specific proteins, receptors, enzymes, genes. Each issue of the journal contains a series of timely in-depth, reviews, original research articles, drug clinical trial studies and guest edited thematic issues written by leaders in the field covering a range of current topics on drug targets involved in cardiovascular and hematological disorders. As the discovery, identification, characterization and validation of novel human drug targets for cardiovascular and hematological drug discovery continues to grow; this journal is essential reading for all pharmaceutical scientists involved in drug discovery and development.

http://www.eurekaselect.com/125080/issue/3

Abstracted & Indexed in

MEDLINE/Index Medicus, EMBASE/Excerpta Medica, Scopus, Chemical Abstracts, BIOSIS Reviews Reports and Meetings, BIOSIS Previews, BIOSIS, BIOBASE, CSA, MediaFinder®-Standard Periodical Directory, Genamics JournalSeek, PubsHub, and J-Gate.

For details about the latest issues, visit:http://benthamscience.com/journal/index.php?journalID=chddt

 

Current Drug Targets, 15 Issue 11

Aims & scope

Current Drug Targets aims to cover the latest and most outstanding developments on the medicinal chemistry and pharmacology of molecular drug targets e.g. disease specific proteins, receptors, enzymes, genes.

Current Drug Targets publishes guest edited thematic issues written by leaders in the field covering a range of current topics of drug targets. The journal also accepts for publication mini- & full-length review articles and drug clinical trial studies.

As the discovery, identification, characterization and validation of novel human drug targets for drug discovery continues to grow; this journal is essential reading for all pharmaceutical scientists involved in drug discovery and development.

http://www.eurekaselect.com/125212/issue/11

Abstracted & Indexed in

Chemical Abstracts, BIOSIS Previews, BIOSIS Reviews Reports and Meetings, Science Citation Index Expanded® (Sci Search), Index to Scientific Reviews®, Biochemistry & Biophysics Citation Index®, PUBMED, BIOSIS, Journal Citation Reports/Science Edition, EMBASE/Excerpta Medica, MediaFinder-Standard Periodical Directory, Medline, Scopus, EMBASE, Genamics Journal Seek, PubsHub, and J-Gate.

For details about the latest issues, visit: http://benthamscience.com/journal/index.php?journalID=cdt

 

Current Medical Imaging Reviews, 10 Issue 3

Aims & scope

Current Medical Imaging Reviews publishes frontier review articles, original research articles, drug clinical trial studies and guest edited thematic issues on all the latest advances on medical imaging dedicated to clinical research. All relevant areas are covered by the journal, including advances in the diagnosis, instrumentation and therapeutic applications related to all modern medical imaging techniques.

The journal is essential reading for all clinicians and researchers involved in medical imaging and diagnosis.

http://www.eurekaselect.com/124511/issue/1

Abstracted & Indexed in

Chemical Abstracts, EMBASE, Scopus, EMNursing, Current Contents/Clinical Medicine, Index to Scientific Reviews, Science Citation Index Expanded, JCR/Science Edition, Genamics JournalSeek, MediaFinder®-Standard Periodical Directory, PubsHub, and J-Gate.

For details about the latest issues, visit: http://benthamscience.com/journal/index.php?journalID=cmir