Press Release for EurekAlert! Probiotics: Novel biosynthetic tool to develop metallic nanoparticles

This research article by Dr. Nida Akhtar et al has been published in Recent Patents on Drug Delivery & Formulation, Volume 11, Issue 1, 2017


Probiotics, being live microbes, exert numerous beneficial health effects on the host cells. Such probiotics are commercially available as dietary supplements, foods, pharmaceutical formulations. Yakult, Activia yogurt, DanActive fermented milk provide health benefits like boosting up the immune system, treating digestive problems, mental illness, neurological disorders, cancer, etc. However, the use of probiotic bacteria to develop metallic nanoparticles (MNPs) constitutes novel research nowadays. Research inputs and patent reports according to an article published in the journal Recent Patents on Drug Delivery and Formulation highlight their potential in the field of cosmetics, pharmaceuticals, medicine and biotechnology as well.

Prof. Kamla Pathak, the corresponding author said: “Our review findings reinstate the potential of probiotic bacteria to produce MNPs. Patents (US, EP and WIPO patents) during 2009-2016 reaffirm the research and inspire the scientific community worldwide for future exploration. This is the first review article in this aspect that systematically tracks the intellectual aspects of applications and synthesis of MNPs by probiotic bacteria. Prof. Pathak said, “Our study details the interventions on which research on MNPs is on. Gold, silver, magnetic, selenium, iron oxide, magnetic-optical iron oxide-gold core-shell, magnesium oxide, copper oxide, aluminum oxide, titanium dioxide, zinc oxide nanoparticles have applications ranging from their usage in analytical methodologies to treat and diagnose diseases. Specifically, gold NPs are suggested for drug delivery and treating lymphocytic leukemia; silver and copper NPs for antimicrobial activity and zinc oxide NPs as anti-corrosive, antifungal and as an additive in food products such as breakfast cereals. These NPs need to be explored more as no patent till date was found on them based on their biosynthesis, according to the author’s report.

For more information about the article, please visit:

Editor’s Choice Article: The Regulation of miRNAs in Inflammation-Related Carcinogenesis

Affiliation: State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China.

Abstract: Chronic inflammation plays important roles in the initiation and development of various cancers, particularly gastrointestinal cancer. Cancer is characterized by stepwise accumulation of genetic and epigenetic alterations of genes. As a high risk factor for cancer, chronic inflammatory response produces great amount of mediators, including cytokines, reactive oxygen and nitrogen species, proteinases, which can induce genetic and epigenetic changes of cancer-associated genes and pathways. Furthermore, inflammation also modulates the expression of miRNAs that not only regulate the expression of tumor-related proteins but also enhance the tumor-promoting inflammatory process. In the current review, we summarize the mechanisms by which inflammatory mediators and signaling regulate the biosynthesis of miRNAs, as well as the involvement of miRNAs in the feedback loops promoting inflammation-associated carcinogenesis.

Contribution by Indian Authors, in BSP Journal: Current Nanoscience


1. Olanzapine Loaded Cationic Solid Lipid Nanoparticles for Improved Oral Bioavailability

Author(s): Sumeet Sood, Natarajan Jawahar, Kunal Jain, Kuppusamy Gowthamarajan and Subramania Nainar Meyyanathan

Affiliation: Dept. of Pharmaceutics, JSS College of Pharmacy, Ootacamund, Tamilnadu-643001, India.


Olanzapine, a lipophilic antipsychotic drug, has poor oral bioavailability due to hepatic first-pass metabolism. Solid Lipid Nanoparticles (SLNs) of Olanzapine were developed using lipids (Stearic acid and Glyceryl monostearate), soy lecithin, poloxamer 188 and charge modifier stearyl amine by microemulsion technique. The aim of this research was to find out whether the bioavailability of olanzapine can be improved by administering olanzapine SLN orally to Wistar rats. Area under curve was increased (up to 4-fold) and clearance was decreased when olanzapine entrapped in SLNs with stearylamine were administered orally compared with that of olanzapine suspension. The enhanced relative bioavailability by the SLNs formulation might be attributed to avoidance of first-pass hepatic metabolism by intestinal lymphatic transport, direct uptake of nanoparticles through the GI tract, increased permeability by surfactants, and decreased degradation and clearance. These results indicate that olanzapine can be loaded into solid lipid nanoparticles for improvement of its oral bioavailability.

2. Extra- and Intracellular Gold Nanoparticles Synthesis Using Live Peanut Callus Cells

Author(s): D. Raju, Urmil J. Mehta and Absar Ahmad

Affiliation: Biochemical Sciences Division and Plant Tissue Culture Division, National Chemical Laboratory, Pune – 411008, Maharashtra, India.


An important challenge of considerable topical significance in nanotechnology is the development of eco-friendly experimental processes for the synthesis of nanomaterials in large quantities with variable sizes, shapes and chemical compositions. Green synthesis routes for the production of inorganic metal nanoparticles using whole cell of microorganisms and plant extracts are gaining tremendous popularity as these are non-toxic, cheap and occur at ambient conditions. The present work emphasizes on gold nanoparticles synthesis protocol using live plant callus cells. Peanut callus cells when incubated with HAuCl4 solution in ambient conditions reduced the precursor and lead to formation of well dispersed, water soluble extracellular and intracellular gold nanoparticles within 24 hours. The biosynthesis of gold nanoparticles was monitored by UV-visible spectroscopy (UV-Vis) and further characterized by X-ray diffraction analysis (XRD), Energy Dispersive Spectroscopy (EDS) and Selected Area Electron Diffraction (SAED). The particle size distribution shows that the average particle size is 50 nm for extra- and 31 nm for intracellular gold nanoparticles. The nanoparticles may be stabilized by proteins secreted by callus cells. The reduction process is believed to occur enzymatically, thus creating the possibility of a rational, plant cell-based method for the synthesis of nanoparticles over a wide range of chemical compositions.

Nanoparticulate Carrier Mediated Intranasal Delivery of Insulin for the Restoration of Memory Signaling in Alzheimer’s Disease

Author(s): Pankaj Dwivedi, Rakesh Kumar Tekade and Narendra Kumar Jain

Affiliation: Pharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar 470 003, India.


The present study was aimed to determine the therapeutic potential of novel carriers to deliver insulin into brain, by passing the BBB. PLGA nanoparticles and PEGylated PLGA nanoparticles were prepared by double emulsification method. PEG-PLGA copolymer was synthesized and characterized by FTIR, NMR and Mass spectroscopies. The release profiles of drug in various formulations were studied in PBS (pH 7.4). Results showed more sustained release of drug with Tween-80 based formulation in comparison with Tween-20 and PVA based formulations. A more sustained and extended release was observed upon chitosan coating of PEG-PLGA nanoparticles. Blood glucose level monitoring suggested that glucose level was not decreased significally in the peripheral region (p>0.05), when chitosan coated insulin loaded PEGylated nanoparticle was administered by intranasal route. This outcome in particular along with expected mucoadhesive and targeted benefit associated with chitosan based formulation drove us to conclude this formulation to be working best for the undertaken brain delivery issue.

For details on our journal, please click on: Current Nanoscience
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