MOST ACCESSED ARTICLE – Genome-wide Analysis of Alternative Splicing in An Inbred Cabbage (Brassica oleracea L.) Line ‘HO’ in Response to Heat Stress

Journal Name: Current Genomics

Author(s): Sang Sook Lee, Won Yong Jung, Hyun Ji Park, Arum Lee, Suk-Yoon Kwon ,Hyun-Soon Kim*, Hye Sun Cho*.




Graphical Abstract:




Introduction: High-throughput RNA sequencing (RNA-Seq) studies demonstrate that Alternative Splicing (AS) is a widespread mechanism that enhances transcriptome diversity, particularly in plants exposed to environmental stress. In an attempt to determine the transcriptome and AS patterns of cabbage inbred line “HO” under Heat Stress (HS), RNA-Seq was carried out using HS-treated and control samples. Genome-wide analysis indicated that AS is differentially regulated in response to HS. The number of AS events markedly increased in HS-treated samples compared to the control.

Conclusion: We identified 1,864 genes, including Heat shock transcription factor (Hsf) and heat shock protein (Hsp) genes, that exhibited >4-fold changes in expression upon exposure to HS. The enriched Gene Ontology (GO) terms of the 1,864 genes included ‘response to stress/abiotic stimulus/ chemical stimulus’, among, which the genes most highly induced by HS encode small Hsps and Hsf proteins. The heat-induced genes also showed an increased number of AS events under HS conditions. In addition, the distribution of AS types was altered under HS conditions, as the level of Intron Retention (IR) decreased, whereas other types of AS increased, under these conditions. Severe HSinduced AS was also observed in Hsfs and Hsps, which play crucial roles in regulating heat tolerance. Our results support the notion that AS of HS-related genes, such as HsfA2 and HsfB2a, are important for heat stress adaptation in cabbage.



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Vegetables Which Mean ‘All Nutrition’

It is a well-known fact that vegetables have loads to offer in terms of health and nutrition. But it appears to be a much generalized statement giving every veg equal score. That certainly is not the case, for we have a vast variety of vegetables in all shapes, colors and sizes. So let’s narrow it down to five vegetables that belong to the same family, i.e. Cruciferae; these high-in-nutrition vegetables are cauliflower, cabbage, broccoli, Brussels sprouts and kale.


These are ranked as the Fantastic Five for the following nutritional benefits they have for us:

  1. Vitamins abundance – cruciferous vegetables are packed with vitamins A, C, K and B group. Vitamin A, as we know is good for eyesight, skin and other organs. Vitamin C boosts the immune system and helps fight off bacterial attacks. Vitamin K is essential for clotting of blood. Vitamin B group has its own benefits, especially for women’s health.
  2. Fibrous veg – All these are rich in fiber, and fiber is useful in clearing the gut and keeping the bowel movement healthy.
  3. Nutrients preventing cancer – there are certain nutrients that can only be obtained through consuming vegetables. These nutrients essentially help in keeping various cancers at bay. Our favorite fives are oozing with these nutrients.
  4. Source of calcium – cruciferous vegetables also have reserve of calcium. Added advantage is that it is present with vitamin K which is crucial in absorbing calcium. As we are moving away from dairy products we can still get calcified through these veggies.
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