Editors Choice Article | Trends in the Analysis of Biopharmaceuticals by HPLC

 

Journal Name: Current Analytical Chemistry

Author(s): Angela Tartaglia, Marcello Locatelli, Victoria Samanidou*.

 

 

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Abstract:

Background: Biopharmaceuticals are biological drugs consisting of a complex compound that can be produced by a living organism or derive from it. Biopharmaceuticals are very complicated compounds from structural point of view and for this reason, they cannot be fully characterized in terms of their structure with current analytical methods as it happens instead of low molecular weight chemicals drugs.

Introduction: The regulatory guidelines require the characterization of the primary or higher sequence of these molecules and the characterization of any post-translational modifications. The use of biopharmaceuticals has really grown in the last few years: in 2016, the number of biopharmaceuticals approved by the Food and Drug Administration (FDA) and European Medicines Agency (EMA) for use in humans’ diseases was 1357. From 2013 to 2016, 73 of these compounds were approved for the treatment of cancer, inflammation, immune disorders, infections, anemia and cardiovascular diseases.

Aim/Conclusion: The aim of the present review is to provide an overview of recent approaches for the characterization of biopharmaceutical products in HPLC that have been presented in the literature in the last years. To read out more, please visit: http://www.eurekaselect.com/168075/article

Podcast | Simultaneous Determination of Substance P and CGRP in Rat Brainstem Tissue Using LC-ESI-MS/MS Method

Author(s): Neva Alasağ*

Journal Name: Current Analytical Chemistry

 

For article details, please visit: https://bit.ly/2HtsTS7

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Aims & Scope | Current Analytical Chemistry

 

Current Analytical Chemistry publishes expert reviews and original research articles on the most recent advances in analytical chemistry. All aspects of the field are represented, including analytical methodology, techniques, and instrumentation in both fundamental and applied research topics of interest to the broad readership of the journal. Current Analytical Chemistry strives to serve as an authoritative source of information in analytical chemistry and in related applications such as biochemical analysis, pharmaceutical research, quantitative biological imaging, novel sensors, and nanotechnology.

 

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Most Accessed Articles | Colorimetric Sensing of Iodide by the Competitive Interactions in the Surface of Gold Nanoparticles with the Simultaneous Aggregation/ Anti-Aggregation Mechanisms in Edible Salts

Author(s): Zolaikha Rasouli, Raouf Ghavami*.

 

Iodide (I-) is a vital micronutrient and its essential roles have been approved in a number of neurological activities. Therefore, there is a growing interest in developing reliable sensitive quantitative methodologies for the determination of the amount of I- in edible salt samples.

First, 2heDTC was synthesized by the mixing of equivalent amounts of pure C2H7NO and CS2 in the presence of NH4OH under vigorous stirring in an ice bath. Then, AuNPs were synthesized; the 250 mL of 1 mM HAuCl4 was put into a round-bottomed flask and was boiled vigorously by stirring for 20 min. Then, 25 mL of 38.8 mM of Na3C6H5O7 was added rapidly into this solution. The solution was heated under reflux for another 15 min. The modification of the AuNPs surface was performed with 2heDTC by proceeding with the ligand exchange reaction. To the quantification of I-, 2 µL (1mM) Hg2+ was added to 0.5 mL (50 mM) of PBS solution with pH = 5.0 containing 500 µL of 2heDTC-AuNPs, which was then maintained at optimized time for the creation of bare AuNPs. Then, the different concentrations of I- were separately added. Finally, the surface plasmon resonance (SPR) absorption bands were taken with UV-Vis spectrophotometer. Read out full article here: http://www.eurekaselect.com/161194

 

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Open Access Articles | Simultaneous Quantification of Citalopram and its Main Metabolite, Desmethylcitalopram, in Human Saliva by UHPLC

Author(s): Ewelina Dziurkowska, Marek Wesolowski*.

Journal Name: Current Analytical Chemistry

This study was designed to develop a reliable method for simultaneous quantitation of Citalopram (CIT) and its main active metabolite, Desmethylcitalopram (DCIT), in saliva of patients undergoing treatment with CIT.

To compare two procedures of saliva purification, Solid-Phase (SPE) and Liquid-Liquid (LLE) extractions, saliva samples obtained from healthy volunteers were spiked with adequate quantities of CIT and DCIT. Different cartridges were used for SPE, while dichloromethane for LLE. Chromatographic separation and quantitation were carried out by UHPLC with DAD detector using a C-18 column and a mixture of acetonitrile and redistilled water (37:63, v:v) with the addition of formic acid (pH 3.5) as a mobile phase. Read out full article here: http://www.eurekaselect.com/157115

 

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Editor’s Choice Article | Graphene Quantum Dots in Electrochemical Sensors/Biosensors

Author(s): Farnoush Faridbod*, Afsaneh L. Sanati.

 

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Graphene and its derivatives, as most promising carbonic nanomaterials have been widely used in design and making electrochemical sensors and biosensors. Graphene quantum dots are one of the members of this family which have been mostly known as fluorescent nanomaterials and found extensive applications due to their remarkable optical properties. Quantum confinement and edge effects in their structures also cause extraordinary electrochemical properties. Read out full article here: http://www.eurekaselect.com/160545/article

New Issue :: Current Analytical Chemistry (Volume: 14, Issue: 5)

 

Current Analytical Chemistry publishes expert reviews and original research articles on the most recent advances in analytical chemistry. All aspects of the field are represented, including analytical methodology, techniques, and instrumentation in both fundamental and applied research topics of interest to the broad readership of the journal. Current Analytical Chemistry strives to serve as an authoritative source of information in analytical chemistry and in related applications such as biochemical analysis, pharmaceutical research, quantitative biological imaging, novel sensors, and nanotechnology.

 

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Articles from the journal Current Analytical Chemistry Volume 14, Issue 5:

 

For details on the articles, please visit this link ::  https://bit.ly/2NJAwJ7

New Issue :: Current Analytical Chemistry (Volume: 14, Issue: 4)

 

Current Analytical Chemistry publishes expert reviews and original research articles on the most recent advances in analytical chemistry. All aspects of the field are represented, including analytical methodology, techniques, and instrumentation in both fundamental and applied research topics of interest to the broad readership of the journal. Current Analytical Chemistry strives to serve as an authoritative source of information in analytical chemistry and in related applications such as biochemical analysis, pharmaceutical research, quantitative biological imaging, novel sensors, and nanotechnology.

 

Articles from the journal Current Analytical Chemistry Volume 14, Issue 4:

For details on the articles, please visit this link ::  https://bit.ly/2KSq2pc

UPCOMING THEMATIC ISSUE – ANALYTICAL NANOSTRUCTURE SENSORS FOR FOOD, PHARMACEUTICAL AND ENVIRONMENTAL ANALYSIS – CURRENT ANALYTICAL CHEMISTRY

CAC -THEMATIC FLYER-Dr. Fatemehh Karimi

https://benthamscience.com/journals/current-analytical-chemistry/

EDITOR’S CHOICE – Therapeutic Monitoring of Carbamazepine in Epilepsy Patients by Highly Sensitive LC/MS Method and its Clinical Applications – Current Analytical Chemistry

Journal: Current Analytical Chemistry

Author(s): Abdul Sami Shaikh, Fanlong Bu, Huanjun Liu, Chunmei Geng, Pingli Li, Rui Zhang, Ruichen Guo*

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Abstract:

Background: Carbamazepine (CBZ) has complex pharmacokinetic properties leading to fluctuation in plasma level, which requires routine therapeutic drug monitoring (TDM) to ensure its efficacy and prevent adverse effects. The objective of this study is directed towards developing and validating a simple, precise, rapid and highly sensitive liquid chromatography-mass spectrometry (LC/MS) technique for measuring CBZ concentration in human plasma of epileptic patients in routine TDM.

Methods: The blood samples of 103 epileptic patients were collected for therapeutic monitoring of CBZ with validated LC/MS assay. The analytes from plasma were extracted with methanol by simple protein precipitation method. The Agilent 1100 LC/MS system was used. The ammonium acetate 5mM: methanol (40: 60 v/v) and diamonsil C18 (150mm×4.6mm, 5µm) were used as mobile phase and analytical column for the separation of analyte, respectively. The temperature of column was 25°C and the rate of flow was set at 0.8 mL/minute. International Conference on Harmonisation (ICH) guidelines were used for the validation of the method.

Results: The stated LC/MS assay displayed good linearity in the range of 5-1000ng/ml and linearity equation of calibration curve was y= 2.44516x +0.00348175 with a correlation coefficient (R2) of 0.99881. The lower limit of quantitation (LLOQ) of the stated technique was observed at concentration of 5ng/ml. The linearity, recovery, accuracy and precision, specificity and stability results were within the acceptance limits of ICH guidelines.

Conclusion: The simple, precise, rapid and highly sensitive LC/MS technique was developed and validated and successfully applied in TDM of 103 epileptic patients who were using CBZ. Besides TDM, the stated method can also be applied in bioequivalence, pharmacokinetics and pharmacovigilance studies.

Read more here: http://www.eurekaselect.com/152187/article

 

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