Open Access Articles – A Sensitive Chromatographic Method for Hyaluronate Quantification Applied to Analyze the Desorption Behavior on Contact Lenses

Journal Name: Current Pharmaceutical Analysis

Author(s): Wan-Hsin Chang, Pei-Yi Liu, Yuan-Ting Jiang*, Yuan-Hao Howard Hsu*.

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Abstract:

Background: Sodium hyaluronate (NaHA) is generally supplemented in products related to contact lenses for increasing comfort during wearing. The quantity of sodium hyaluronate and the material of lenses affect the retention of sodium hyaluronate on the contact lenses.

Methods: We developed a convenient and sensitive but unconventional chromatographic method to quantify sodium hyaluronate and analyze its release behavior from contact lenses. The reverse-phase chromatography eluted sodium hyaluronate with high molecular masses in the shortest time and could separate salt and small compounds from sodium hyaluronate.

Results: This method could accurately quantify sodium hyaluronate with diverse molecular sizes. Because sodium hyaluronate was eluted in a narrow time frame, sensitivity was significantly enhanced, and the limit of detection of this method was 0.45 μg/mL. According to this quantitation method, the attached quantity of sodium hyaluronate is related to the water content of the material. Furthermore, a material test indicated that the release efficiency of sodium hyaluronate depends on the material of lenses. Nonionic Polymacon had a longer half-life in the sodium hyaluronate release curve than negative Methafilcon A and silicone hydrogel.

Conclusion: This hyaluronate quantification method is a fast, sensitive and accurate method, making it suitable for the in vitro hyaluronate research without further derivatization. To read out more, please visit: http://www.eurekaselect.com/170419/article

New Issue – Current Pharmaceutical Analysis; Volume 16 Issue 1

AIMS & SCOPE

Current Pharmaceutical Analysis publishes full-length/mini reviews and original research articles on all the most recent advances in pharmaceutical and biomedical analysis. All aspects of the field are represented including drug analysis, analytical methodology and instrumentation. The journal is essential to all involved in pharmaceutical, biochemical and clinical analysis.

Articles from the Journal 

• An Easy and Rapid Spectrophotometric Method for Determination of Chloroquine Diphosphate in Tablets
• Development and Validation of a Specific RP-HPLC Method for Simultaneous Estimation of Anti-retroviral Drugs: Application to Nanoparticulate Formulation System
• Quantification and Evaluation of Glycyrrhizic Acid-loaded Surface Decorated Nanoparticles by UHPLC-MS/MS and used in the Treatment of Cerebral Ischemia
• In vitro Identification of Spinosin Metabolites in Human Liver Microsomes Using a Simple and Sensitive UHPLC-Q-TOF-MS/MS Method
• A Sensitive Microscale HPLC-UV Method for the Determination of Doxofylline and its Metabolites in Plasma: An Adapted Method for Therapeutic Drug Monitoring in Children
• Stability-indicating UPLC-PDA Method for Ambrisentan Tablets and Identification of a Main Degradation Product by UPLC-MS/MS
• Simultaneous Determination of Hydrochlorothiazide in Combination with Some Antihypertensive Drugs in The Presence of Its Main Impurities in Pure Form and Pharmaceutical Formulations
• Quantitative Determination of Ginsenoside Rg1 in Rat Plasma by Ultrahigh Performance Liquid Chromatography-tandem Mass Spectrometry (UHPLC-MS/MS) and its Application in a Pharmacokinetics and Bioavailability Study
• An LC-MS Method for Determination of Betamethasone in Tissue Engineering Skin and Application to Dermatopharmacokinetic Study
• Simultaneous Determination of Cholecalciferol and 25- Hydroxycholecalceferol in Lipid-based Self-nanoemulsifying formulations and Marketed Product Vi-de 3® by UHPLC-UV
• Quantification of Genotoxic Impurities in Key Starting Material of Sertraline Hydrochloride by Simple and Sensitive Liquid Chromatography Technique

For more details on the articles, please visit here

New Issue | Current Pharmaceutical Analysis; Volume 16 Issue 3

Current Pharmaceutical Analysis

Volume 16, Issue 3

AIMS & SCOPE

Current Pharmaceutical Analysis publishes full-length/mini reviews and original research articles on all the most recent advances in pharmaceutical and biomedical analysis. All aspects of the field are represented including drug analysis, analytical methodology and instrumentation. The journal is essential to all involved in pharmaceutical, biochemical and clinical analysis.

 

Articles from the Journal 

• LC-MS/MS Determination of Evodiamine in Plasma and Its Pharmacokinetics in Rats after Administration in the Form of Solid Lipid Nanoparticles
• Development and Validation of an RP-HPLC Method for Determination of Atorvastatin and its Hydroxyl Metabolites in Human Plasma
• Comparison of UV- and Derivative-Spectrophotometric and HPTLC UVDensitometric Methods for the Determination of Amrinone and Milrinone in Bulk Drugs
• A Smart Green Spectrophotometric Method for Simultaneous Determination of Severely Overlapped Binary Mixtures Using Normalized Spectrum and Isosbestic Point as Resolving Tools
• Identification of Candidate Biomarkers for HCV Leading to Hepatocellular Carcinoma Differential Stages From Serum Samples
• Development and Validation of an LC-MS/MS Method for Quantitative Determination of EAI045, A Novel EGFR Inhibitor, in Rat Plasma
• Simultaneous Determination of 17 Constituents of Chinese Wild Radix Salvia miltiorrhiza from Different Geographical Areas by Ultra-High Performance Liquid Chromatography Coupled to Triple Quadrupole Mass Spectrometry
• Advanced High-Coverage Targeted Metabolomics Method (SWATHtoMRM) for Exploring the Relationship of Follicular Fluid Componen ts with Age
• New Monitoring Strategy for the Quality Control in the Processing Practice of Scutellariae Radix
• Voltammetric Method Development for Itopride Assay in a Pharmaceutical Formulation
• The Optimization of HL60-IL6 Assay and its Application in the Pyrogen Detection of Monoclonal Antibody

 

For more details on the articles, please visit HERE 

 

 

 

 

 

 

 

Editors Choice Article | Advanced High-Coverage Targeted Metabolomics Method (SWATHtoMRM) for Exploring the Relationship of Follicular Fluid Componen ts with Age

Journal Name: Current Pharmaceutical Analysis

Author(s): Jingyan Song, Tianqi Wang, Jiayin Guo, Ying Guo, Xiaoming Wang, Yi Yang, Kaiyue Xu, Yuanhong Sa, Lihua Yuan, Huaying Jiang, Zhengao Sun*.

 

Abstract:

Background: The complexity of follicular fluid metabolome presents a huge challenge for qualitative and quantitative metabolite profiling and discovery of the comprehensive biomarkers.

Objective: In order to address this challenge, novel SWATHtoMRM metabolomics method was used for providing broad coverage and excellent quantitative capability to discover the human follicular fluid metabolites related to age and evaluate their relationship with pregnancy outcome and oocyte senescence.

Methods: The patients were divided into four groups according to age, including group A (28 cases, 21- 27 years old), group B (42 cases, 28-34 years old), group C (31 cases, 35-41 years old), and group D (24 cases, 42-48 years old). Follicular fluid samples from 125 IVF patients were analyzed. The differential ions among the four groups were identified by principal components analysis according to accurate mass, isotope ratio, and tandem mass spectroscopic spectra. Then, the differential metabolic pathways were further identified by a KEGG cluster analysis.

Results: A total of 18 metabolites in the follicular fluid differed among the four groups, including amino acids, lipids, hormones, and vitamins. A total of 15 metabolites, including 6-oxohexanoate, phenylalanine, proline, hexadecanoic acid, linoleate, arachidonate, oleic acid, docosahexaenoic acid, LysoPC(16:1), LysoPC(20:5), LysoPC (20:3), 25-hydroxyvitamin D3, 5-dehydroepisterol, 27- hydroxycholesterol, and 5beta-cholestane-3alpha,7alpha,12alpha,23,25-pentol, were down-regulated with age and 3 metabolites, including LysoPC(18:3), LysoPC(18:1), and 13,14-dihydroretinol, were upregulated with age.

Conclusion: Our study provides useful information for revealing the relationship between age and female reproductive capability.

 

 

 

To read out more, please visit: http://www.eurekaselect.com/170068/article

 

 

Editors Choice Article | In vitro Identification of Spinosin Metabolites in Human Liver Microsomes Using a Simple and Sensitive UHPLC-Q-TOF-MS/MS Method

Journal Name: Current Pharmaceutical Analysis

Author(s): Qiaoyue Zhang, Xia Zhang, Yanyan Liu, Changchen Wan, Yupeng Sun, Lantong Zhang*.

 

 

 

 

Graphical Abstract:

 

Abstract:

Background: Spinosin is one of the major bioactive constituents among the total flavonoids in semen ziziphi spinosae, which has sedation and hypnosis actions.

Methods: A simple and rapid high-resolution ultra-high-performance liquid chromatography coupled with a quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) method was developed and validated for predicting the structures of its spinosin metabolic products. This paper presents the first research focused on the metabolites of spinosin in human liver microsomes.

Results: Based on the analytical strategy, 8 spinosin metabolites were detected in human liver microsome incubation samples, and the metabolic pathways required to generate these metabolites were proposed. However, no phase II metabolites were found. The cytochrome P450 enzyme is the main metabolic enzyme involved in drug metabolism, accounting for approximately 75% of the total number of different metabolic reactions.

Conclusion: The in vitro metabolism of spinosin was proposed. These results allow us to learn about spinosin metabolism, leading to a better understanding of drug biotransformation and providing a basis for clinical applications. Moreover, this study laid the foundation for developing new pharmaceutical drugs. To read out more, please visit: http://www.eurekaselect.com/165902/article

Open Access Articles | Tissue Distribution of Active Principles from Alpiniae Oxyphyllae Fructus Extract: An Experimental Study in Rats

 

Journal Name: Current Pharmaceutical Analysis

            Author(s): Qi Wen, Hai-Long Li, Shi-Ying Mai, Yin-Feng Tan*, Feng Chen*.

 

Graphical Abstract:

 

Abstract:

Background: Alpiniae Oxyphyllae Fructus (Yizhi in Chinese) have been widely used as an herbal medicine for the treatment of diuresis, enuresis and diarrhea in China. Many studies have deciphered some potential underlying mechanisms for its anti-diarrheal effects. However, tissue distribution of Yizhi constituents is warranted because pharmacological receptors are frequently located in tissues. Moreover, it is also interesting to know about the potential correlation between behavior in drug distribution and the observed pharmacological response. The aim of this study is to investigate tissue distribution behaviors of Yizhi constituents after oral administration of Yizhi extract to rats, focusing on 10 active principles.

Methods: Twenty four male Sprague Dawley rats were given orally the Yizhi extract and fourteen tissue samples were collected after being killed by bleeding from the abdominal aorta under ether anesthesia at different time-points. The resulting tissues were excised and homogenized. Based on our previous reports, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to quantify the target analytes, as well as phase II metabolites, in the various biosamples.

Results: Almost all the targeted Yizhi active principles and some glucuronidated metabolites were qualitatively measured in rat stomach, small intestine, large intestine, as well as liver. Nootkatone, yakuchinone A and tectochrysin were observed in the rat brain. In other rat tissues, these analytes had lower exposure or could not be detected. Consistently, quantitative analysis revealed that the Yizhi active principles dominantly distributed into gastrointestinal tissues followed by liver, the overall exposure levels ranking as follows: stomach > small intestine > large intestine > liver. Tissue concentrationtime profiles of the test active principles in rat stomach, small intestine, and large intestine were bimodal with two concentration peaks occurring at 0.5 and 4h after oral administration, respectively. The exposure levels in rat kidney and bladder were quite low.

Conclusion: The active principles of Yizhi were specially distributed into gastrointestinal tissues after oral administration of its ethanol extract to rats. The tissue distribution behaviors partly supported its anti-diarrheal effects from a pharmacokinetic opinion. This paper will be useful as the starting point for studying the pharmacological activities of this traditional herb. To read out more, please visit: http://www.eurekaselect.com/165218/article

Aims & Scope | Current Pharmaceutical Analysis

 

AIMS & SCOPE

Current Pharmaceutical Analysis publishes full-length/mini reviews and original research articles on all the most recent advances in pharmaceutical and biomedical analysis. All aspects of the field are represented including drug analysis, analytical methodology and instrumentation. The journal is essential to all involved in pharmaceutical, biochemical and clinical analysis.

 

Wishing you a very Happy Birthday | Dr. Anastasios Economou

 

Dr. Anastasios Economou

Editor in Chief: Current Pharmaceutical Analysis

University of Athens
Athens
Greece

New Issues of various Bentham Science Journals

 

1. Mini-Reviews in Medicinal Chemistry, Vol: 18, Issue: 16

https://bit.ly/2qCXROI

2. Mini-Reviews in Medicinal Chemistry, Vol: 18, Issue: 17

https://bit.ly/2qBcaDd

3. Current Drug Targets, Vol: 19, Issue: 13

https://bit.ly/2QttYLL

4. Current Drug Metabolism, Vol: 19, Issue: 10

https://bit.ly/2qAdypw

5. Current Cancer Drug Targets, Vol: 18, Issue: 8

https://bit.ly/2z3e6sN

6. Current Pharmaceutical Analysis, Vol: 14, Issue: 6

https://bit.ly/2PNXYVE

7. The Natural Products Journal, Vol: 8, Issue: 3

https://bit.ly/2yYGJY6

8. Current Organocatalysis, Vol: 5, Issue: 2

https://bit.ly/2RKY65t

9. Current Drug Safety, Vol: 13, Issue: 3

https://bit.ly/2OvKMjC

10. Current Topics in Medicinal Chemistry, Vol: 18, Issue: 12

https://bit.ly/2D9ecSA

 

EDITOR’S CHOICE – Advances in Anti-Doping Analytical Approaches – Current Pharmaceutical Analysis

Journal: Current Pharmaceutical Analysis

Author(s): Xianchi Li, Peiying Zhang*

Graphical Abstract:

 

Abstract:

Background: Performance enhancement substances and methods other than exercise training and physical conditioning have become a major problem in athletic competitions. Over the last few years, there has been an increase in the number of these doping approaches used by some athletes, including pharmaceuticals, slightly modified endogenous compounds and also blood transfusions. In order to control and prevent these doping practices by athletes, World Anti-Doping Agency has stipulated several guidelines and approved various methods on the basis of reproducibility, sensitivity and adaptability. The number, design and type of doping substances are increasing on daily basis necessitating the rapid development of analytical methods to detect these substances and to prevent doping.

Objective: In this review, we address the various methodological developments in the last few years to track down the novel doping substances as well as doping methods.

Results: There have been significant advances in the area of mass spectroscopy and the associated detection devices to measure small quantities of test substance or their metabolites in body tissues and fluids. Some of the doping substances have short biological half-life but leave imprints of their action in the form of altered gene expression, protein expression or metabolism, which can be detected by OMICs technologies.

Conclusion: The rapid advance in biological instrumentation and our understanding of the molecular basis of the actions of doping substances have paved the way to enforce ‘true play’ in athletic competitions. But this is an incessant and a continuous process as long as the doping practices continue.

Read more here: http://www.eurekaselect.com/151469/article