As the proteomics technology develops and human genomic analysis becomes easier to execute, molecules involved in the regulation of biochemical signaling pathways have become interesting subjects for researchers. Earlier research was based on assumptions that metabolic cascades such as phosphorylation was linear. However, current studies indicate a multi-network signaling cascade. The interlinked signals performing in synchrony can be observed being responsive to a stimulus during physiological regulation. Although phosphatase is not considered as an inert house-keeping enzyme in the process of phosphorylation and regulation, its catalytic and regulatory actions show an involvement in signal transduction pathways. The key role of phosphatases is now defined to maintain and regulate the degree of signal’s intervals. According to recent studies, Shp2, a phosphatase protein has shown to have a remarkable role in governing the signaling response amplitude.